WANG, Xiaojuan（Associate Professor）

  Name：

WANG, Xiaojuan

Academic Title：

Associate Professor

Advisor Type：

Supervisor for Mater Students 

  Department：

Department of Applied Chemistry

Research Interests：

The conjugation, assembly and application of Functional Nanomaterials

  E-Mail：

xwang@upc.edu.cn

  Telephone：

(0532) 86981560

◎Educational Background

1995， B.S. in Chemistry, Shandong University, Jinan, China,

2004， Ph.D. in Chemistry, University of Basel, Basel, Switzerland,

◎Work Experience

2005-2009，Postdoctoral Researcher at Department of Chemical Engineering and Biotechnology, Cambridge University, Cambridge, U.K.

2009-2011，Research Scientist at Nanoco Technologies Ltd, Manchester, U.K.

2011-present，Associate Professor at the University of Petroleum (Huadong), Qingdao, China

◎Research Direction

[1] Surface modification of nanomaterials according to the application directions, such as the photodegradation of pollutants, light-emitting devices, chemical analysis, disease diagnosis and treatment.

[2] Exploring the interaction mechanism and application of various molecules, including organic polymers, biomacromolecules, inorganic ions, and nanoparticles.

◎Research Project

[1] Evaluation of the application effect of seaweed-based carbon nanocomposite photocatalysts for the degradation of polychlorinated biphenyls in soil;

[2] Construction of intelligent short peptide-modified fluorescent gold nanoclusters and functional development of labeling nucleoli and carrying nucleic acid fragments;

[3] The effect and mechanism of graphene quantum dots on molecular chaperone-mediated protein folding;

[4] Fluorescence scintillation carbon dot super-resolution imaging of the aggregation and distribution of G protein-coupled receptors on cell membranes;

[5] Key technology of plasma nano-coating detection chip for early warning of major malignant tumors;

[6] Using polypeptide-modified cadmium-free quantum dots as fluorescent probes to study the movement of CXCR4 on cell membranes;

[7] Application of cadmium-free quantum dots in light-emitting diode technology.

◎Representative Papers and Patents

1. Paper

[1]X. Wang, Z. Zhang, Z. Liu, X. Ma, Q. Dai, X. Wang, B. Ge, H. He, F. Huang. Spectroscopic investigation on the binding interactions between graphene quantum dots and carbonic anhydrase. Spectrochim Acta, Part A. 2022;265:120369.

[2]刘彩, 刘芳, 黄方, 王晓娟. 海藻基cds-cu-tio₂复合材料的制备及其光催化性能. 无机材料学报. 2021;36(11):1154-1162.

[3]Q. Wang, H. He, Q. Zhang, Z. Feng, J. Li, X. Chen, L. Liu, X. Wang, B. Ge, D. Yu, H. Ren, F. Huang. Deep-learning-assisted single-molecule tracking on a live cell membrane. Anal Chem. 2021;93(25):8810-8816.

[4]Q. Wang, Z. Feng, H. He, X. Hu, J. Mao, X. Chen, L. Liu, X. Wei, D. Liu, S. Bi, X. Wang, B. Ge, D. Yu, F. Huang. Nonblinking carbon dots for imaging and tracking receptors on a live cell membrane. Chem Commun. 2021;57(45):5554-5557.

[5]X. Ma, X. Wang, C. Liu, B. Ge, H. He, Q. Dai, Z. Zhang, J. Yu, W.M. Nau, F. Huang. Self-assembled theranostic microcarrier targeting tumor cells with high metastatic potential. Materials & Design. 2021;212:110196.

[6]J. Li, Y. Ding, H. Liu, H. He, D. Yu, X. Wang, X. Wang, X. Yu, B. Ge, F. Huang. Oligomerization-enhanced receptor-ligand binding revealed by dual-color simultaneous tracking on living cell membranes. The journal of physical chemistry letters. 2021;12:8164-8169.

[7]H. He, L. Liu, X. Chen, Q. Wang, X. Wang, W.M. Nau, F. Huang. Carbon dot blinking enables accurate molecular counting at nanoscale resolution. Anal Chem. 2021;93(8):3968-3975.

[8]Q. Dai, X. Wang, C. Liu, Z. Feng, B. Ge, X. Ma, Z. Zhang, J. Yu, X. Wang, F. Huang. Detection of tyrosinase in living cells using an enteromorpha prolifera based fluorescent probe. Anal Chim Acta. 2021;1169:338605.

[9]Y. Xia, S. Gao, H. He, X. Wang, Z. Zeng, B. Chen, N. Zou, M. Cao, S. Wang. A new and straightforward strategy to prepare an optical hydrogel film with dynamic structural colors. The Journal of Physical Chemistry C. 2020;124(29):16083-16089.

[10]X. Ma, X. Wang, Q. Chen, H. He, Y. Sun, H. Liu, Y. Wang, J. Qu, F. Huang. Glycosaminoglycan / gold nanocluster hybrid nanoparticles as a new sensing platform: Metastatic potential assessment of cancer cells. Carbohydr Polym. 2020;230:115654.

[11]H. He, X. Shi, J. Wang, X. Wang, Q. Wang, D. Yu, B. Ge, X. Zhang, F. Huang. Reactive oxygen species-induced aggregation of nanozymes for neuron injury. ACS Appl Mater Interfaces. 2020;12(1):209-216.

[12]Y. Wang, X. Wang, X. Ma, Q. Chen, H. He, W.M. Nau, F. Huang. Coassembly of gold nanoclusters with nucleic acids: Sensing, bioimaging, and gene transfection. Part Part Syst Charact. 2019;36(10):1900281.

[13]X. Wang, Z. Liu, Q. Chen, X. Wang, F. Huang. Interactions between graphene materials and proteins. Progress in Chemistry. 2019;31(2-3):236-244.

[14]X. Wang, Q. Chen, Z. Zhang, H. He, X. Ma, Z. Liu, B. Ge, F. Huang. Novel enteromorpha prolifera based carbon dots: Probing the radical scavenging of natural phenolic compounds. Colloids Surf, B. 2019;174:161-167.

[15]S. Wang, B. Cui, Q. Cai, Y. Bu, X. Wang, M. Cao, Y. Xia, H. He. Fabrication of highly luminescent sio2–au nanostructures and their application in detection of trace hg2+. J Mater Sci. 2019;54(10):7517-7528.

[16]G. Jiang, X. Li, Y. Che, Y. Lv, F. Liu, Y. Wang, C. Zhao, X. Wang. Antibacterial and anticorrosive properties of cuzno@rgo waterborne polyurethane coating in circulating cooling water. Environmental Science and Pollution Research. 2019;26(9):9027-9040.

[17]Y. Xia, D. Tang, H. Wu, X. Wang, M. Cao, H. He, S. Wang. Cell attachment/detachment behavior on poly(n-isopropylacrylamide)-based microgel films: The effect of microgel structure and swelling ratio. J Mater Sci. 2018;53(12):8795-8806.

[18]Y. Song, B. Ge, J. Lao, Z. Wang, B. Yang, X. Wang, H. He, J. Li, F. Huang. Regulation of the oligomeric status of ccr3 with binding ligands revealed by single-molecule fluorescence imaging. Biochemistry (Mosc). 2018;57(5):852-860.

[19]N.U. Khan, H. He, X. Wang, B. Ge, Q. Wang, X. Liu, J. Lao, Y. Wang, J. Li, Z. Wang, S. Zhou, F. Huang. A two-color fluorescence enhanced dot-blot assay for revealing co-operative expression of chemokine receptors in cells. Chem Commun. 2018;54(7):778-781.

[20]N.U. Khan, Z. Feng, H. He, Q. Wang, X. Liu, S. Li, X. Shi, X. Wang, B. Ge, F. Huang. A facile plasmonic silver needle for fluorescence-enhanced detection of tumor markers. Anal Chim Acta. 2018;1040:120-127.

[21]M.H. Jacob, R.N. D'souza, T. Schwarzlose, X. Wang, F. Huang, E. Haas, W.M. Nau. Method-unifying view of loop-formation kinetics in peptide and protein folding. J Phys Chem B. 2018;122(16):4445-4456.

[22]H. He, X. Wang, F. Huang. Analysis and applications of single-molecule fluorescence in live cell membranes. In: Wang H, Li G, editors. Membrane biophysics: Springer; 2018. p. 147-173.

[23]H. He, S. Li, X. Shi, X. Wang, X. Liu, Q. Wang, A. Guo, B. Ge, N.U. Khan, F. Huang. Quantitative nanoscopy of small blinking graphene nanocarriers in drug delivery. Bioconjug Chem. 2018;29(11):3658-3666.

[24]H. Chi, B. Xu, Z. Liu, J. Wei, S. Li, H. Ren, Y. Xu, X. Lu, X. Wang, X. Wang, F. Huang. Combined thermodynamic and kinetic analysis of groel interacting with cxcr4 transmembrane peptides. Biochim Biophys Acta. 2018;1862:1576-1583.

[25]Y. Xia, Y. Tang, H. Wu, J. Zhang, Z. Li, F. Pan, S. Wang, X. Wang, H. Xu, J.R. Lu. Fabrication of patterned thermoresponsive microgel strips on cell-adherent background and their application for cell sheet recovery. ACS Appl Mater Interfaces. 2017;9(2):1255-1262.

[26]X. Wang, Y. Wang, H. He, X. Ma, Q. Chen, S. Zhang, B. Ge, S. Wang, W.M. Nau, F. Huang. Deep-red fluorescent gold nanoclusters for nucleoli staining: Real-time monitoring of the nucleolar dynamics in reverse transformation of malignant cells. ACS Appl Mater Interfaces. 2017;9(21):17799-17806.

[27]J.-B. Qu, Y.-L. Xu, Y. Liu, Y. Wang, Y. Sui, J.-G. Liu, X. Wang. Inherently fluorescent polystyrene microspheres for coating, sensing and cellular imaging. Colloids Surf, B. 2017;152:475-481.

[28]J. Lao, H. He, X. Wang, Z. Wang, Y. Song, B. Yang, N. Ullahkhan, B. Ge, F. Huang. Single-molecule imaging demonstrates ligand regulation of the oligomeric status of cxcr4 in living cells. J Phys Chem B. 2017;121(7):1466-1474.

[29]H. He, X. Liu, S. Li, X. Wang, Q. Wang, J. Li, J. Wang, H. Ren, B. Ge, S. Wang, X. Zhang, F. Huang. High-density super-resolution localization imaging with blinking carbon dots. Anal Chem. 2017;89(21):11831-11838.

[30]P. Zhao, M. Yang, W. Fan, X. Wang, F. Tang, C. Yang, X. Dou, S. Li, Y. Wang, Y. Cao. Facile one-pot conversion of petroleum asphaltene to high quality green fluorescent graphene quantum dots and their application in cell imaging. Part Part Syst Charact. 2016;33(9):635-644.

[31]Y. Xu, J. Qu, X. Wang, J. Liu, S. Li, J. Liu. Preparation of polystyrene-based fluorescent microspheres and their luminescence mechanism. Acta Polym Sin. 2016(10):1368-1373.

[32]X. Wang, Y. Wang, H. He, X. Chen, X. Sun, Y. Sun, G. Zhou, H. Xu, F. Huang. Steering graphene quantum dots in living cells: Lighting up the nucleolus. J Mater Chem B. 2016;4(4):779-784.

[33]X. Wang, H. He, Y. Wang, J. Wang, X. Sun, H. Xu, W.M. Nau, X. Zhang, F. Huang. Active tumor-targeting luminescent gold clusters with efficient urinary excretion. Chem Commun. 2016;52(59):9232-9235.

[34]H. He, Z. Wang, T. Cheng, X. Liu, X. Wang, J. Wang, H. Ren, Y. Sun, Y. Song, J. Yang, Y. Xia, S. Wang, X. Zhang, F. Huang. Visible and near-infrared dual-emission carbogenic small molecular complex with high rna selectivity and renal clearance for nucleolus and tumor imaging. ACS Appl Mater Interfaces. 2016;8(42):28529-28537.

[35]H. He, X. Wang, T. Cheng, Y. Xia, J. Lao, B. Ge, H. Ren, N.U. Khan, F. Huang. An ultra-high fluorescence enhancement and high throughput assay for revealing expression and internalization of chemokine receptor cxcr4. Chem--Eur J. 2016;22(17):5863-5867.

[36]X.-D. Zhang, J. Chen, J. Yang, J.-Y. Wang, X. Shen, S.-S. Song, H. Wang, H. He, X. Wang, S. Fan, Y.-M. Sun, M. Guo. Use of epidermal growth factor receptor antibody-gold cluster conjugates with good renal excretion in targeted cancer radiation treatment. J Mater Chem B. 2015;3(23):4735-4741.

[37]T. Yue, X. Wang, X. Zhang, F. Huang. Molecular modeling of interaction between lipid monolayer and graphene nanosheets: Implications for pulmonary nanotoxicity and pulmonary drug delivery. RSC Adv. 2015;5(38):30092-30106.

[38]X. Wang, X. Sun, H. He, H. Yang, J. Lao, Y. Song, Y. Xia, H. Xu, X. Zhang, F. Huang. A two-component active targeting theranostic agent based on graphene quantum dots. J Mater Chem B. 2015;3:3583-3590.

2. Patent

[1] Wang Xiaojuan, Huang Fang, Wang Yanan, Qu Jianbo, He Hua, Wang Shengjie, A KCK polypeptide-modified gold nanocluster and its preparation method, 201610387321.0

[2] Xiaojuan Wang, Jianbo Qu, Yanan Wang, Fang Huang, Hua He, Shengjie Wang, A peptide-modified gold nanocluster and its preparation method, 201610389189.7

[3] Wang Xiaojuan, Chen Qi, Zhang Zhengyuan, Huang Fang, Wang Shengjie, Pan Siyuan, Ge Baosheng, He Hua, Preparation method and application of fluorescent carbon quantum dots using seaweed as carbon source, 201611128316.4

[4] Wang Xiaojuan, Ma Xiqi, Wang Yanan, Huang Fang, Ge Baosheng, He Hua, A gene carrier system and its construction method, 201711350421.7

[5] Wang Xiaojuan, Chen Qi, Huang Fang, Ge Baosheng, Liu Zhenzhen, A method for the determination of phenolic antioxidants using carbon quantum dots as fluorescent indicators, 201711350390.5

[6] Wang Shengjie, Wang Xiaojuan, Jing Qian, Cao Meiwen, Xia Yongqing, A preparation method of graphene dispersion, 201711379802.8

[7] Wang Xiaojuan, Ma Xiqi, Huang Fang, Ge Baosheng, Sun Yixin, A method for the detection of polysulfate chondroitin impurities in heparin sodium, 201711430491.3

[8] Wang Xiaojuan, Dai Qi, Sun Yixin, Qu Jianbo, Huang Fang, Zhang Zhixiong, A tyrosinase detection probe based on carbon quantum dots and its preparation method and application, 201911162539.6

[9] Wang Xiaojuan, Yu Jinyi, Liu Hui, Huang Fang, Dai Qi, A preparation method of multifunctional liposome, 202010709982.7

[10] Wang Xiaojuan, Zhang Zhixiong, Ma Xiqi, Werner M. Nuo, Huang Fang, Dai Qi, A method for intracellular delivery of proteins, 202010960117.X

[11] Wang Xiaojuan, Liu Cai, Liu Fang, Huang Fang, Ge Baosheng, Dai Qi, A preparation method of biomimetic synthetic activated carbon-titanium dioxide composite, 202011203083.6

◎Courses Offered

Undergraduate：《Modern Instrumental Analysis》《Biophysical Chemistry》《Specific English for Applied Chemistry》《Clothing, Garment Accessories, and Chemistry 》

Postgraduate / Foreign Students：《Advanced Biochemistry》《Biochemical Instrumental Analysis》

◎Student Training

1. Supervise Postgraduate Students

Co-guided 1 doctoral student, guided 17 academic master students and 6 professional master students. Till 2021, 8 students have graduated, of which 2 went abroad for doctoral study, 3 worked in institutions, and 3 went to companies to undertake R&D work.

2. Typical student：

SUN Xing, a Master student under my supervision between 2012-15, published 5 SCI papers during his Master degree study, won the Roberto Roca Scholarship in 2014, and returned to China to work in Hunan University after finishing his PhD at Marburg University in Germany in 2020;

WANG Yanan, a Master student under my supervision between 2014-17, published 5 SCI papers during her Master degree study, won Chinese National Scholarship for excellent graduates in 2016, and went to study at the University of Geneva in Switzerland for PhD in 2017. In 2022, she continued her postdoctoral research at the University of Michigan in USA.

DAI Qi, a Master student joined in from 2019, has published 3 SCI papers (3 papers are being submitted), won Chinese National Scholarship in 2021, and will go to Sichuan University to continue his doctoral study this summer.

3. Admissions Instructions：

Academic Master students majored in Chemical Engineering and Technology (081700)

Professional Master students majored in Chemical Engineering (085602)