Hua He（Associate professor）

  Name：

Hua He

Academic Title：

Associate professor

Advisor Type：

MSc Supervisor

  Department：

Biology and Energy Chemical   Engineering

Research Interests：

Bioanalytical Chemistry

  E-Mail：

huahe@upc.edu.cn

  Telephone：

0532-86981135

◎Educational Background

1998-2002，BS, College of Chemistry and Chemical   engineering, Hunan Normal University   

2002-2005，MSc, College of Chemistry and Chemical   engineering, Hunan Normal University

2005-2008，PhD College of Chemistry and Chemical   engineering, Shanghai Jiaotong University

2014-2015，Visiting scholar, Department of   Chemistry, Stanford University

◎Work Experience

2008-2010，Assistant Professor, College of Chemistry   and Chemical engineering，China University of Petroleum (East China)

2010-Present, Associate   Professor, College of Chemistry and Chemical engineering, China University of   Petroleum (East China)

◎Research Direction

[1] Single molecule/particle/cell fluorescence   imaging analysis

[2] Design, synthesis and biomedical   application of functional nanoprobes

[3] Surface-enhanced fluorescence   imaging for molecular detection and in vitro diagnosis

◎Research Project

[1] National Natural Science Foundation   of China (grant nos. 21874154, 20905078)

[2] National Natural Science Foundation   of Shandong (grant nos. ZR2014BM028，ZR2009BQ001)

[3] Fundamental Research Funds for the   Central Universities (grant nos. 18CX02126A,12CX04053A)

◎Representative Papers and Patents

1. Paper

  [1]    Qian Wang, Qian Zhang, Hua   He,* Zhenzhen Feng, Jian Mao, Xiang Hu, Xiaoyun Wei, Si-min   Bi, Guangyong Qin, Xiaojuan Wang, Baosheng Ge, Daoyong Yu, Hao Ren and Fang   Huang* Carbon Dot Blinking Fingerprint Uncovers Native Membrane Receptor   Organizations via Deep Learning. Analytical   Chemistry 2022, 94(9), February 21.

  [2]    X. Mu, J. Wang, Hua He   (co-first), Qifeng Li, Bing Yang, Junhui Wang, Haile Liu,   Yalong Gao, Lufei Ouyang, Si Sun, Qinjuan Ren, Xinjian Shi, Wenting Hao,   Qiaoman Fei, Jiang Yang, Lulin Li, Ryan Vest, Tony Wyss-Coray, Jian Luo, and   Xiao-Dong Zhang*.An oligomeric semiconducting nanozyme with ultrafast electron transfers   alleviates acute brain injury.Science Advances2021, 7(46), eabk1210

  [3]    Hua He,* Xiaoliang Chen, Zhenzhen   Feng, Lihua Liu, Qian Wang, and Simin Bi.Nanoscopic Imaging of   Nucleolar Stress Enabled by Protein-Mimicking Carbon Dots. Nano Letters2021, 21(13),   5689–5696

  [4]    Naseer Ullah Khan, Muhammad   Zahir, Xukun Liu, Jing Lin, Qihong Zheng, Huajie Zhang, Samiullah Malik, Hua   He ,* and Liming Shen.* Ultra-sensitive detection of exosome   using biofunctionalized gold nanorods on silver-island film. Nano Letters 2021, 21(13),   5532–5539.

  [5]    Qian wang, Hua He,*   Qian Zhang, Zhenzhen Feng, Jiqiang Li, Xiaoliang Chen, Lihua Liu, Xiaojuan   Wang, Baosheng Ge, Daoyong Yu, Hao Ren and Fang Huang* Deep-Learning-Assisted   Single-Molecule Tracking on a Live Cell Membrane. Analytical Chemistry 2021, 93(25), 8810–8816.

  [6]    Hua He, Lihua Liu, Xiaoliang Chen,   Qian wang, Xiaojuan Wang, Werner M. Nau, and Fang Huang.Carbon Dot Blinking Enables   Accurate Molecular Counting at Nanoscale Resolution. Analytical Chemistry 2021, 93(8), 3968–3975.

  [7]    Qian wang, Zhenzhen Feng, Hua   He,* Jiqiang Li, Xiaoliang Chen, Lihua Liu, Xiaojuan Wang,   Baosheng Ge, Daoyong Yu, Hao Ren and Fang Huang*.Nonblinking Carbon Dots for   Imaging and Tracking Receptors on Live Cell membrane. Chemical Communications 2021, 57(45), 5554-5557.

  [8]    Hua He,* Di Liu, Zhenzhen Feng, Aijun Guo, Lihua   Liu, and Xiaoliang Chen.Antifade Carbon Dots on Plasmonic Substrate for   Enhanced Protein Detection in Immunotherapy. ACS Sensors 2020, 5(12), 4027-4034.

  [9]    Hua He, Xinjian Shi, Junying Wang, Xiaojuan Wang,   Qian Wang, Daoyong Yu, Baosheng Ge, Xiaodong Zhang and Fang Huang. Reactive   Oxygen Species-Induced Aggregation of Nanozymes for Neuron Injury. ACS Applied Materials & Interfaces   2020,12(1), 209-216.

[10]      Lufei Ouyang, Xiaoyu Mu, Junying Wang, Qifeng Li,   Yalong Gao, Haile Liu, Si Sun, Qinjuan Ren,     Ruijuan Yan, Jingya Wang, Qiang Liu, Yuanming Sun, Changlong Liu,   Hua He,* Wei Long and Xiaodong Zhang*. Carbon dot targeting to   nitrogen signaling molecules for inhibiting neuronal death. Journal of Materials Chemistry B 2020,8(11), 2321-2330.

[11]      Xiaoyu Mu, Hua He (co-first), Junying Wang, Wei Long, Qifeng Li, Haile Liu,   Yalong Gao, Lufei Ouyang, Qinjuan Ren, Si Sun, Jingya Wang, Jiang Yang, Qiang   Liu, Yuanming Sun, Changlong Liu, XiaoDong Zhang, Wenping Hu. Carbogenic   Nanozyme with Ultrahigh RNS Selectivity for Traumatic Brain Injury. Nano Letters2019, 19(7),   4527-4534.

[12]      Naseer Ullah Khan, Hua He*, Xiaojuan   Wang, Baosheng Ge, Qian Wang, Xu Liu, Jun Lao, Yanan Wang, Jiqiang Li,   Zhencai Wang, Shitan Zhou, and Fang Huang*. A Two-Color Fluorescence Enhanced   Dot-Blot Assay for Revealing Co-operative Expression of Chemokine Receptors   in Cells. Chemical Communications 2018,   54, 778-781.

[13]      Hua He, Shan Li, Xinjian Shi, Xiaojuan Wang, Xu Liu,   Qian Wang, Aijun Guo, Baosheng Ge, Naseer Ullah Khan, and Fang Huang*, Quantitative   Nanoscopy of Small Blinking Graphene Nanocarriers in Drug Delivery. Bioconjugate Chemistry2018,   29 (11), 3658–3666.

[14]      Naseer Ullah Khan, Zhenzhen Feng, Hua He*,   Qian Wang, Xu Liu, Shan Li, Xinjian Shi, Xiaojuan Wang, Baosheng Ge and Fang   Huang*, A Facile Plasmonic Silver Needle for Fluorescence-Enhanced Detection   of Tumor Markers. Analytica Chimica   Acta2018, 1040, 120-127.

[15]      Hua He, Xu Liu, Shan Li, Xiaojuan Wang, Qian Wang,   Jiqiang Li, Junying Wang, Hao Ren, Baosheng Ge, Shengjie Wang, Xiaodong   Zhang, Fang Huang, High-Density Super-Resolution Localization Imaging with   Blinking Carbon Dots. Analytical   Chemistry2017, 89 (21), 11831-11838.

[16]      Jun Lao, Hua He (co-first), Xiaojuan Wang, Zhencai Wang, Yanzhuo Song, Bin   Yang, Naseer Ullahkhan, Baosheng Ge*, Fang Huang*. Single-Molecule Imaging   Demonstrates Ligand Regulation of Oligomeric Status of CXCR4 in Living Cells.   ‎The   Journal of Physical Chemistry B 2017, 121 (7), 1466-1474.

[17]      Hua He, Zhencai Wang, Tiantian Cheng, Xu Liu, Xiaojuan   Wang, Junying Wang, Hao Ren, Yawei Sun, Yanzhuo Song, Jiang Yang, Yongqing   Xia, Shengjie Wang, Xiaodong Zhang*, and Fang Huang*. Visible and   Near-Infrared Dual-Emission Carbogenic Small Molecular Complex with High RNA   Selectivity and Renal Clearance for Nucleolus and Tumor Imaging. ACS Applied Materials & Interfaces   2016, 8 (42), 28529-28537.

[18]      Hua He, Xiaojuan Wang, Tiantian Cheng, Yongqing Xia, Jun   Lao, Baosheng Ge, Hao Ren, Naseer Ullah Khan, Fang Huang*. An Ultra-High   Fluorescence Enhancement and High Throughput Assay for Revealing Expression   and Internalization of Chemokine Receptor CXCR4. Chemistry-A European Journal 2016, 22, 5863-5867.

[19]      Hua He*, Xiaojuan Wang, Zhenzhen   Feng, Tiantian Cheng, Xing Sun, Yawei Sun, Yongqing Xia, Shengjie Wang,   Junying Wang, Xiaodong Zhang*. Rapid microwave-assisted synthesis of   ultra-bright fluorescent carbon dots for live cell staining, cell-specific   targeting and in vivo imaging. Journal   of Materials Chemistry B2015, 3, 4786-4789.

[20]      Hua He, Xing Sun, Xiaojuan Wang, Yawei, Sun, Hai Xu*,   Jian R. Lu*. Cyclic Arginyl-Glycyl-Aspartic Acid (RGD) Peptide-Induced   Synthesis of Uniform and Stable One-Dimensional CdTe Nanostructures in Aqueous   Solution. RSC Advances 2014,   4, 11794-11797.

[21]      Hua He, Min Feng, Jing Hu, Cuixia Chen, Jiqian Wang,   Xiaojuan Wang, Hai Xu*, Jian R. Lu*. Designed Short RGD Peptides for One-Pot   Aqueous Synthesis of Integrin-binding CdTe and CdZnTe Quantum Dots. ACS Applied Materials & Interfaces2012, 4, 6362-6370.

[22]      Hua He, Chao Xie, Jicun Ren*. Non-Bleaching Fluorescence   of Gold Nanoparticles and Its Applications in Cancer Cell Imaging. Analytical Chemistry2008,   80(15): 5951-5957.

[23]      Hua He, Jicun Ren*. A Novel Evanescent Wave Scattering   Imaging Method for Single Gold Particle Tracking in Solution and on Cell   Membrane. Talanta2008,   77(1):166-171.

[24]      Hua He, Huifeng Qian, Chaoqing Dong, Kanglin Wang, Jicun   Ren*. Single nonblinking CdTe quantum dots synthesized in aqueous   thiopropionic acid. Angewandte   Chemie International Edition 2006, 45(45): 7588-7591.

[25]      Hua He, Qingji Xie, Shouzhuo Yao*. An electrochemical   quartz crystal impedance study on anti-human immunoglobulin G immobilization   in the polymer grown during dopamine oxidation at an Au electrode.Journal of Colloid and Interface Science2005, 289, 446-454.

◎Representative Works

[1] Hua   He, Xiaojuan Wang, Fang Huang. Analysis and   Applications of Single Molecule Fluorescence in Live Cell Membranes, in:   ‎Membrane Biophysics: New Insights and Methods, ‎Springer2018, pp 147-173.

◎Awards and Honors

[1] Third prize of Qingdao Natural   Science Award in 2008

[2] Outstanding graduates of Shanghai   Jiaotong University in 2008

[3] Hong Kong Qiushi Postgraduate   Scholarship in 2008“

[4] National excellent scholarship in   2007

[5] Outstanding graduate (postgraduate)   of Hunan Normal University in 2005

[6] Outstanding graduate (undergraduate)   of Hunan Normal University in 2002

◎Courses Offered

Undergraduate：《Biophysical Chemistry》,《Materials and Health》,《Materials and Environment》

Postgraduate：《Instrumental Analysis   Technology and Application》,《Biomaterials》,《Bioinstrumental Analysis Technology》

◎Student Training

1.Supervise Postgraduate Students

3 PhD students, 2 academic postgraduates   and 13 professional postgraduates.

2.Admissions Instructions：

Biochemistry, Bioengineering, Biology   and Medicine

◎Part-time Academic Job

[1] Frontiers in chemistry editorial   board

[2] Evaluation expert of medical   engineering combination fund of Tianjin University